The procedure is carefully controlled for temperature and timing, with incubations typically ranging from 1 to 2 h at room temperature to overnight settings at 4C, depending on the target analyte
Handa K, Inukai K, Onuma H, Kudo A, Nakagawa F, Tsugawa K,
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Incompatibility : Concomitant use with strong acids, strongly oxidizing agents, or in environments with an excessively acidic pH is not recommended, as these conditions could degrade the molecule and alter the distinctive blue color of the finished cosmetic