These proteins are: horse heart apocytochrome c (Stellwagen et al., 1972), protein phosphatase inhibitor-1 from rabbit skeletal muscle (19.2 kDa by sedimentation and 60.0 kDa by gel-filtration) (Nimmo and Cohen, 1978), a dopamine-and adenosine 3:5-monophosphate-regulated phosphoprotein from bovine caudate nucleus, DARPP-32 (27.6 kDa by sedimentation and 59 kDa by gel-filtration) (Hemmings et al., 1984), bovine cardiac troponin C in the absence of calcium (McCubbin and Kay, 1985), heat-stable microtubule-associated protein MAP2 (Hernandez et al., 1986), smooth muscle caldesmon (Lynch et al., 1987), brain-specific 14-kDa protein (14 kDa by Sodium Dodecyl Sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 57 kDa by gel filtration) (Nakajo et al., 1990), carboxyl-terminal transactivation domain of Vmw65 from herpes simplex virus type 1 (11.0 kDa from sedimentation and 55.0 kDa from gel-filtration) (Donaldson and Capone, 1992), prothymosin (Stokes radius is 1.77 larger than those expected for a compactly folded protein consisting of 109 amino acid residues) (Gast et al., 1995), chromogranin B (100 kDa by SDS-PAGE and 220 kDa by gel-filtration) (Yoo, 1995), human topoisomerase I (66 kDa by sedimentation and 300 kDa by gel-filtration) (Stewart et al., 1996), N-terminal domain of human topoisomerase I (198 residues, 66 kDa by sedimentation and 96 kDa by gel filtration) (Stewart et al., 1996), oncoprotein 18 (Op18)/stathmin from fetal calf thymus (17.22 kDa by mass-spectrometry and 64 kDa by gel-filtration) (Schubart et al., 1987
For non-standard ratios, separate vials are the right tool
Spheres were then washed once in PBS and imaged in fresh PBS on an Olympus IX83 Inverted Microscope (Mason Technology) using the TRITC (Ex/Em: 396/580 nm) and DAPI (Ex/Em: 353/483) filter sets
Oxidative stress and redox imbalance : Accumulation of reactive oxygen species (ROS) and a decrease in antioxidant activity disrupts mitochondrial functions and promotes cellular damage
