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Cellular viability assays Cells were seeded in 384-well plates at an approximate number of 6001000 cells per well

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For estimation of the importance of glucagon receptors, we used mice with global knockout of Gcgr ( Gcgr / ) [22], and regarding the GLP-1 receptor we used the GLP-1 receptor antagonist exendin 9-39 (Ex9)

A recent systematic review of oral probiotic interventions in metabolic disorders reported modest but consistent improvements in glycemic parameters, including fasting glucose and HbA1c, alongside signals of enhanced incretin activity, supporting a mechanistic link between probiotic supplementation and gut hormone regulation (109)