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An iScript cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to amplify 1 g of RNA per reaction in a MyCycler thermal cycler (Bio-Rad)

In the current study, the estimated V max and K m were 0.23 nmol/min/mg and 86.05 mol/L, while Murcia and Diaz reported that GSTs extracted from liver of Rhode Island Red chickens displayed V max as 1.40 pmol/min/mg and K m as 65.66 mol/L in the formation of AFBO-GSH [31]

Your skin also darkens due to Tyrosinase, an enzyme that darkens your skin due to oxidation and melanin biosynthesis

In islet , brain, and renal tubular cells, the reaction is mainly directed towards oxidative deamination of glutamate due to high glutamate and low -KG/NH 3 levels [5]