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Studies comparing GHK (apo) vs GHK-Cu consistently show reduced activity for the copper-free peptide

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H&E staining results further confirmed that the aortic wall thickness of mice treated with FOXO4-DRI was significantly thinner, and ultrasound examination results showed that the PWV of the aorta was slowed down after FOXO4-DRI treatment, indicating better vascular elasticity and significantly improved vascular function compared to the control group (Figures 2D,E)

This regulatory effect on the HPA axis may contribute to its anxiolytic and sleep-promoting properties