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Observation on altered hepatocellular foci in National Toxicology Program 2-year carcinogenicity studies in rats

Decreases in the levels of citrate, creatinine, isoleucine, hydroxyphenyllactate, and glutamate were also found suggesting roles for altered branched-chain amino acid metabolism (isoleucine, hydroxyphenyllactate) and abnormal mitochondrial energy production (succinic acid, DHEA-S, citrate, aspartate, glutamate)

The standard curve method was used to determine mRNA concentrations, and each gene was normalized to cyclophilin ( Ppia ) expression

The excess of ROS was revealed to lead to an increased oxidative stress level, involved in various cardiovascular pathologies (2-12)