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The resulting BAM files had PCR duplicates, mitochondrial reads, and ENCODE blacklist 107 features removed before further analysis using Picard v3.4.0 (Broad Institute, 2019), samtools v1.13 93 , and bedtools v2.30.0 108 , respectively

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(2020) Identification of the 3-amino-3-carboxypropyl (acp) transferase enzyme responsible for acp3U formation at position 47 in Escherichia coli tRNAs, Nucleic Acids Res

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