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NanoBiT assays were carried out as previously described (28): 24 hours after transfection, cells were incubated in 3 mM glucose for a minimum of 2 hours, detached and resuspended in NanoGlo Live cell substrate (N2012, Promega), 1:20 dilution in HBSS, and seeded onto an opaque white 96-well half-area plate

Flinn RJ, Yan Y, Goswami S, Parker PJ, Backer JM

Hypertension: Elevated blood pressure may occur, increasing the risk of cardiovascular problems

This peptide hormone promotes glucose-dependent insulin secretion, inhibits glucagon secretion, suppresses appetite, and stimulates islet -cell proliferation and inhibits their apoptosis