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For the electron flow assays, mitochondria were plated in MAS buffer with 10 mM pyruvate + 2 mM malate + 4 M FCCP followed by 20 M rotenone in port A, 100 mM succinate in port B, 40 M antimycin A in port C and 1 mM TMPD + 100 mM ascorbic acid in port D
Since temperature can significantly change real-world battery performance, you may also want to see how battery temperature effects performance in different environments
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In addition , it offers a more controlled and consistent tanning approach throughout the year
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