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Semen collection For semen collection, bull ejaculates were collected twice a week for 6 weeks with an artificial vagina pre-warmed to 42C according to the department's protocols (55)

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This is controlled by a complex reflex involving central neural control centres located in the lateral reticular formation of the medulla which are stimulated by the chemoreceptor trigger zones (CTZs) in the floor of the fourth ventricle, and also by vagal afferents from the gut