FIGURE 2 The Light-Sensitive Mutant Combining the Deletion of slr0236 and the Depletion of sll1545 Exposed to High-Light Undergoes a Transient Sequential Accumulation of ROS and Then of Reduced (GSH) and Oxidized (GSSG) Glutathione To analyze the influence of high-light on the mutants slr0236 (absence of slr0236 ), sll1545 (depletion of sll1545 ), slr0236-sll1545 (combined absence of slr0236 and depletion of sll1545 ) and the WT strain we measured the ROS content (Figures 3A,B), using the standard fluorescent probe DCHF-DA ( total = GSH + GSSG) (Figures 3G,H) using the relevant standard assay ( slr0236-sll1545 mutant facing high light (7500 lux) rapidly (in 1 h) accumulated ROS (Figure 3B)
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doi: 10.1177/1098612X12439358
