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Note additionally that the three methionine residues in the sequence, two of them in the N-terminal extension, make dilute standing solution vulnerable to oxidation, and that freeze-thaw cycling promotes aggregation in disulfide-bonded proteins more readily than in short peptides
J Am Soc Nephrol 25(4):784797
303 Heart failure Heart failure (HF) is a clinical illness characterized by structural impairment of the heart, impairing its capacity to deliver adequate blood to satisfy the metabolic requirements of essential organ functions
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