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Another class such as the multipass transmembrane protein design method developed by David Bakers team 46 , which successfully constructed a complex topology containing 8 transmembrane domains by realising the connection of dispersed helices and sequence optimisation through Rosetta

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TB-500 (Thymosin Beta-4 Fragment): Chemical Name: N-Acetyl-L-leucyl-L-lysyl-L-lysyl-L-threonyl-L-alpha-glutamyl-L-threonyl-L-glutamine Amino Acid Sequence: Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH Molecular Formula: C38H68N10O14 Molecular Weight: 889.01 g/mol

KG is derived from multiple metabolic pathways, including oxidative decarboxylation of isocitrate via isocitrate dehydrogenases (IDHs) and glutaminolysis ( Fig