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A florescent probe like 2,7-dichlorofluorescein diacetate (H 2 DCFDA) penetrates the spermatozoa and indicates H 2 O 2 concentrations, as H 2 O 2 de-esterifies in the presence of DCFH and forms fluorescent 2,7-dichlorofluorescein (DCF) ( Dihydroethidium/hydroethidine (DHE) is a non-florescent probe that is oxidized by a variety of reactive oxygen and nitrogen species

D., Feighner, S

Capacitation involves the hyperactivation of sperm motility and the preparation for the acrosome reaction, a critical step in penetrating the oocyte

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