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To disentangle drug adverse effects from inflammatory activity, clinicians must employ a concrete diagnostic approach

For DBS and VAMS just a simple extraction step with organic solvent mixtures is needed prior to HPLC-MS/MS analysis but, depending on the blood volume sampled, lower analytical performance has to be taken into account (Osteresch et al

Notably, ursolic acid has been observed to induce inflammation by enhancing the production of nitric oxide and TNF- in resting macrophages through NF-B transactivation, and upregulates IL-1, an important pro-inflammatory mediator