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These results suggest that both low and high copper levels induce oxidative stress, with HepG2 ATP7B-KO cells being more sensitive under physiological copper conditions

However, a recent study found that strong lipid peroxidation also occurs in atypical pyroptosis, so it should no longer be used as the sole biomarker for validating ferroptosis [17]

Calcium assay in CHO-GLP-1R cells PathHunter GLP-1R cells were loaded with calcium dye (Calcium 6, Molecular Devices) in HBSS plus 20 mM HEPES and 2.5 mM probenecid 1 M agonist for 90 min at 37 C