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The elution of the FLAG-protein complex was conducted using a FLAG peptide

Components of Smoke Disrupt Immune Function Awad adds, We suspected that some of the more than 20,000 chemicals present in cigarette smoke that smokers inhale might also bind to MR1 and influence the activity of MAIT cells in the lungs. The researchers used computer modeling to predict which components of cigarette smoke might be recognized by MR1 and found that several of these molecules not only bound to the protein but also either increased or decreased its amounts on the surface of cells